RNA

Part:BBa_K4065010

Designed by: Raúl Acosta Murillo   Group: iGEM21_FCB-UANL   (2021-09-02)


XylR gRNA guide for CAS9 for Knock out for Bacillus subtilis

RNA guide for XylR gene knock-out, Bacillus subtilis organism and 75% efficiency via CAS9 enzyme designed through Chopchop software Labun, K., Montague, T. G., Krause, M., Torres Cleuren, Y. N., Tjeldnes, H., & Valen, E. CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Nucleic Acids Research (2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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